Description:

Size: 100 microliters

Catalog no.: GENTObsm-2011M-A594

Price: 489 EUR

Product details

Gene ID

590

Clone

1E8

Target Protein/Peptide

BCHE

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Modification

Not modified

Conjugated with

ALEXA FLUOR 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Host organism

Mouse (Mus musculus)

Cross Reactive species

Human (Homo sapiens)

Clonality

Monoclonal Antibodies

Purification method

Purified by Protein G.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Specificity

Highly specific antibody against BCHE

Properties

For facs or microscopy Alexa 1 conjugate.

Cross Reactive Species details

No significant cross reactivity is observed

Antigen Source

Full length native protein purified from human

Alternative name 2

BCHE(1E8) Polyclonal anti-, ALEXA FLUOR 594 Conjugated

Alternative name 1

BCHE(1E8) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated

Storage

Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; CHE1; Butyrylcholine esterase; Pseudocholinesterase; butyrylcholinesterase.CHLE_HUMAN.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Background information

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008].

Advisory

Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.