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Providers / Bioss Monoclonal Antibodies / Anti-TNF-alpha (1F6) Monoclonal, ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObsm-0387M-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Clone

1F6

Gene ID

7124

Applications

IF(IHC-P)

Target Protein/Peptide

TNF-alpha

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Modification

Not modified

Conjugated with

ALEXA FLUOR 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Cross Reactive species

Human (Homo sapiens)

Host organism

Mouse (Mus musculus)

Clonality

Monoclonal Antibodies

Purification method

Purified by Protein G.

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

Highly specific antibody against TNF-alpha

Cross Reactive Species details

No significant cross reactivity is observed

Alternative name 2

TNF-alpha (1F6) Monoclonal anti-, ALEXA FLUOR 594 Conjugated

Alternative name 1

TNF-alpha (1F6) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated

Antigen Source

This antibody is produced using full length recombinant human TNF-alpha.

Storage

Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF

Description

The Anti-TNF-alpha (1F6) Monoclonal, is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Advisory

Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.

Background information

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.