Product details

Clone

2H5

Gene ID

N/A

Applications

IF(IHC-P)

Target Protein/Peptide

Hepatocyte

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Modification

Not modified

Conjugated with

ALEXA FLUOR 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Antigen Source

human liver extract

Cross Reactive species

Human (Homo sapiens)

Host organism

Mouse (Mus musculus)

Clonality

Monoclonal Antibodies

Purification method

Purified by Protein G.

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

Highly specific antibody against Hepatocyte

Cross Reactive Species details

No significant cross reactivity is observed

Alternative name 2

Hepatocyte(2H5) Monoclonal anti-, ALEXA FLUOR 594 Conjugated

Alternative name 1

Hepatocyte(2H5) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated

Storage

Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

Hepatocyte specic antigen; Cell growth inhibiting protein 42; Growth inhibiting protein 20; Human ALB; Human serum albumin.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Advisory

Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.

Background information

Hepatocyte specific antigen is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. Hepatcoyte labels an antigen in the mitochondrial fraction of the liver homogenates. Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes. HSA (Hepatocyte Specific Antigen) has been demonstrated consistently in the vast majority of hepatocellular carcinomas. Studies have shown the utility of HSA in the differential diagnosis of hepatocellular carcinoma, cholangiocarcinoma and hepatoblastomas.

Anti-Hepatocyte(2H5) Monoclonal, ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObsm-2741M-A594

Price: 489 EUR