Swiss Prot
N/A
Anti-NEDD4L (Ser342) (Polyclonal), ALEXA Fluor 594
Description:
Size: 100 microliters
Catalog no.: GENTObs-3299R-A594
Price: 516 EUR
Product details
Subcellular locations
N/A
Gene ID
23327
Modification site
Ser342
Applications
IF(IHC-P)
Concentration
1ug per 1ul
Excitation emission
590nm/617nm
Target Protein/Peptide
NEDD4L Ser342
Modification
Phosphorylation
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Clonality
Polyclonal Antibody
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Other name
Anti-NEDD4L (Ser342) Polyclonal
Also known as
NEDD4L (Ser342) Polyclonal Antibody
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with NEDD4L (Ser342)
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Antigen Source
KLH conjugated synthetic phosphopeptide derived from human NEDD4L around the phosphorylation site of Ser342
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Synonyms
E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; E3 ubiquitin-protein ligase NEDD4-like protein; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; NEDD4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed, developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5.
Background information
E3 ubiquitin-protein ligase accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Furthermore, it promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5, and promotes the ubiquitination and degradation of SGK.