Subcellular locations
N/A
Anti-Haspin (Polyclonal), ALEXA Fluor 594
Description:
Size: 100 microliters
Catalog no.: GENTObs-15413R-A594
Price: 489 EUR
Product details
Swiss Prot
N/A
Modification site
None
Gene ID
83903
Target Protein/Peptide
Haspin
Applications
IF(IHC-P)
Excitation emission
590nm/617nm
Concentration
1ug per 1ul
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Clone
Polyclonal Antibodies
Other name
Anti-Haspin Polyclonal
Purification method
Purified by Protein A.
Group
Polyclonals and antibodies
Also known as
Haspin Polyclonal Antibody
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with Haspin
Modification
No modification has been applied to this antibody
Antigen Source
KLH conjugated synthetic peptide derived from human Haspin
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Synonyms
germ cell associated 2; Germ cell-specic gene 2 protein; GSG2; H-Haspin; Haploid germ cell-specic nuclear protein kinase; HASP_HUMAN; Serine/threonine-protein kinase Haspin.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Background information
Haspins is a protein family containing a distinctive C-terminal kinase domain and a divergent N-terminus. Haspin homologues occur within a diverse group of eukaryotes, including animals, plants and fungi, which suggests an early evolutionary origin. Haspin, a nuclear protein strongly expressed in male germ cells, is responsible for the phosphorylation of histone H3 at Thr-3. Depletion of Haspin RNA prevents normal alignment of chromosomes at metaphase, suggesting a crucial role for haspin during chromosome segregation. Expression of haspin also occurs in adult thymus and bone marrow, with weaker expression in adult prostate, intestine, lung, spleen, and lymph node. The gene encoding human haspin maps to chromosome 17p13.