1ug per 1ul
Alexa conjugate 1
ALEXA FLUOR® 594
Purified by Protein A.
Polyclonals and antibodies
Conjugated Primary Antibody
Alexa Fluor,ALEXA FLUOR® 594
Rabbit (Oryctolagus cuniculus)
FZR1/CDC20C Polyclonal Antibody
For facs or microscopy Alexa 1 conjugate.
No modification has been applied to this antibody
This antibody reacts specifically with FZR1/CDC20C
KLH conjugated synthetic peptide derived from human FZR1/CDC20C
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C; Fizzy-related protein homolog; CDC20-like protein 1; Cdh1/Hct1 homolog; FZR1; FYR; KIAA1242
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.