Swiss Prot
N/A
Anti-CHEK1 (Ser296) (Polyclonal), ALEXA Fluor 594
Description:
Size: 100 microliters
Catalog no.: GENTObs-11226R-A594
Price: 516 EUR
Product details
Gene ID
N/A
Subcellular locations
N/A
Modification site
Ser296
Applications
IF(IHC-P)
Concentration
1ug per 1ul
Excitation emission
590nm/617nm
Target Protein/Peptide
CHEK1 Ser296
Modification
Phosphorylation
Conjugated
Alexa conjugate 1
Conjugated with
ALEXA FLUOR® 594
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clonality
Polyclonal Antibody
Cross reactive species
Human (Homo sapiens)
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Other name
Anti-CHEK1 (Ser296) Polyclonal
Host organism
Rabbit (Oryctolagus cuniculus)
Also known as
CHEK1 (Ser296) Polyclonal Antibody
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with CHEK1 (Ser296)
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Antigen Source
KLH conjugated synthetic phosphopeptide derived from human CHEK1 isoform b around the phosphorylation site of Ser296
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Synonyms
Chk1 Ser296; Chk1 phospho S296;Chk1 Ser296; p-Chk1 phospho S296; Chk1S296; Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog S. pombe; CHEK 1; CHEK-1; CHEK1; Chk 1; Chk1; CHK1 checkpoint homolog S. pombe; EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A S. cerevisiae; C85740; CHK1_HUMAN.
Background information
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011].