Description:

Size: 100 microliters

Catalog no.: GENTObs-9392R-A594

Price: 489 EUR

Product details

Subcellular locations

N/A

Swiss Prot

N/A

Modification site

None

Gene ID

10036

Target Protein/Peptide

p150 CAF1

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-p150 CAF1 Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Also known as

p150 CAF1 Polyclonal Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with p150 CAF1

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human p150 CAF1

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

CAF-1 p150; CAF 1 150 kDa subunit; CAF 1; CAF 1 subunit A; CAF; CAF I 150 kDa subunit; CAF I p150; CAF Ip150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF1 150 kDa subunit; CAF1; CAF1 p150 Subunit; CAF1 subunit A; CAF1A; CAF1A; CAF1A_HUMAN; CAF1B; CAF1P150; CAF1P155; CAF1P155; CHAF1A; Chromatin assembly factor 1 subunit A; Chromatin Assembly Factor 1 Subunit A p150; Chromatin assembly factor I 150 kDa; Chromatin assembly factor I 150 kDa; Chromatin Assembly Factor I; Chromatin assembly factor I p150 subunit; DCAF1; hp150; Nucleosome Remodeling Factor 150kDa Subunit; NURF150; P150.

Background information

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.