Gene ID Number
699
Bub1 Antibody, ALEXA FLUOR 555
Description:
Size: 100ul
Catalog no.: bs-4294R-A555
Price: 380 EUR
Product details
Modification Site
None
Target Antigen
Bub1
Swiss Prot
O43683
Subcellular location
Nucleus
French translation
anticorps
Tested applications
IF(IHC-P)
Clonality
Polyclonal
Modification
Unmodified
Excitation emission
553nm/568nm
Concentration
1ug per 1ul
Immunogen range
980-1030/1085
Crossreactivity
Human, Mouse, Rat
Conjugated with
ALEXA FLUOR® 555
Clone
Polyclonal antibody
Recommended dilutions
IF(IHC-P)(1:50-200)
Purification
Purified by Protein A.
Conjugation
Alexa Fluor,ALEXA FLUOR 555
Also known as
Anti-Bub1 PAb ALEXA FLUOR 555
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Specificity
This is a highly specific antibody against Bub1.
Long name
Bub1 Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
Synonyms
BUB1A; BUB1L; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Bub1
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties
For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Background of the antigen
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.