Gene ID Number
3020
Histone H3 (Tri Methyl K27) Antibody, ALEXA FLUOR ...
Description:
Size: 100ul
Catalog no.: bs-3773R-A350
Price: 350 EUR
Product details
Tested applications
IF(IHC-P)
French translation
anticorps
Clonality
Polyclonal
Modification
Methylation
Excitation emission
343nm/442nm
Concentration
1ug per 1ul
Modification Site
Tri Methyl K27
Conjugated with
ALEXA FLUOR® 350
Crossreactivity
Human, Mouse, Rat
Recommended dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal antibody
Purification
Purified by Protein A.
Target Antigen
Histone H3 Tri Methyl K27
Conjugation
Alexa Fluor,ALEXA FLUOR 350
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Also known as
Anti-Histone H3 Tri Methyl K27 PAb ALEXA FLUOR 350
Long name
Histone H3 (Tri Methyl K27) Antibody, ALEXA FLUOR 350 Conjugated
Specificity
This is a highly specific antibody against Histone H3 Tri Methyl K27.
Synonyms
Histone H3, H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene.
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
KLH conjugated synthetic methyl peptide derived from human Histone H3 around methylation site of Lys27
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
Background of the antigen
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.