Description:

Size: 100ul

Catalog no.: bs-3447R-A488

Price: 350 EUR

Product details

Gene ID Number

4846

Swiss Prot

P29474

Modification Site

Ser1177

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Concentration

1ug per 1ul

Excitation emission

499nm/519nm

Conjugation

Alexa Fluor

Target Antigen

eNOS Ser1177

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 488

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Subcellular location

Cytoplasm, Cell membrane

Crossreactivity

Human, Mouse, Rat, Rabbit

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-eNOS Ser1177 PAb ALEXA FLUOR 488

Specificity

This is a highly specific antibody against eNOS Ser1177.

Long name

eNOS (Ser1177) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser1177

Synonyms

eNOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Background of the antigen

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-eNOS Ser1177 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.