Description:

Size: 100ul

Catalog no.: bs-0573R-A350

Price: 380 EUR

Product details

Modification Site

None

Gene ID Number

25729

Swiss Prot

P39949

Subcellular location

Nucleus

Target Antigen

Cyclin E

French translation

anticorps

Clonality

Polyclonal

Crossreactivity

Mouse, Rat

Modification

Unmodified

Concentration

1ug per 1ul

Excitation emission

343nm/442nm

Immunogen range

370-411/411

Tested applications

FCM, IF(IHC-P)

Conjugated with

ALEXA FLUOR® 350

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR 350

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Cyclin E PAb ALEXA FLUOR 350

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Synonyms

Ccne; CYCLE; G1/S-specific cyclin-E1; Ccne1

Specificity

This is a highly specific antibody against Cyclin E.

Long name

Cyclin E Polyclonal Antibody, ALEXA FLUOR 350 Conjugated

Source

KLH conjugated synthetic peptide derived from rat Cyclin E

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. Transcript Variant: This variant (1) contains a different 5' end region, which includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a 15 aa longer N-terminus, as compared to isoform 2.