Description:

Size: 100ul

Catalog no.: bs-0623R-A488

Price: 380 EUR

Product details

Modification Site

None

Gene ID Number

12443

Swiss Prot

P25322

French translation

anticorps

Target Antigen

Cyclin D1

Clonality

Polyclonal

Immunogen range

70-120/295

Modification

Unmodified

Excitation emission

499nm/519nm

Concentration

1ug per 1ul

Conjugation

Alexa Fluor

Conjugated with

ALEXA FLUOR® 488

Subcellular location

Cytoplasm, Nucleus

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Crossreactivity

Human, Mouse, Rat, Goat

Tested applications

FCM, IF(IHC-P), IF(ICC)

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Cyclin D1 PAb ALEXA FLUOR 488

Specificity

This is a highly specific antibody against Cyclin D1.

Recommended dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Long name

Cyclin D1 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Source

KLH conjugated synthetic peptide derived from mouse Cyclin D1

Synonyms

cD1; CycD1; Cyl-1; PRAD1; bcl-1; AI32739; G1/S-specific cyclin-D1; Ccnd1

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Cyclin D1 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).