Product details

Target Antigen

BrdU

Modification Site

None

Cross-reactive species details

BrdU

Crossreactivity

Others

French translation

anticorps

Tested applications

IF(IHC-P)

Modification

Unmodified

Clonality

Polyclonal

Excitation emission

650nm/665nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 647

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Source

BrdU conjugated to KLH

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR 647

Also known as

Anti-BrdU PAb ALEXA FLUOR 647

Host Organism

Rabbit (Oryctolagus cuniculus)

Specificity

This is a highly specific antibody against BrdU.

Long name

BrdU Polyclonal Antibody, ALEXA FLUOR 647 Conjugated

Synonyms

Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2-deoxyuridine; 5-BrdU; proliferation Marker.

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-BrdU exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

BrdU Antibody, ALEXA FLUOR 647

Description:

Size: 100ul

Catalog no.: bs-0489R-A647

Price: 380 EUR