Description:

Size: 100ul

Catalog no.: bs-3185R-A647

Price: 350 EUR

Product details

Gene ID Number

3014

Swiss Prot

P16104

Modification Site

Ser140

Subcellular location

Nucleus

French translation

anticorps

Tested applications

IF(IHC-P)

Clonality

Polyclonal

Concentration

1ug per 1ul

Excitation emission

650nm/665nm

Immunogen range

100-142/143

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 647

Crossreactivity

Human, Mouse, Rat

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Target Antigen

Histone H2A.X Ser140

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR 647

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Histone H2A.X Ser140 PAb ALEXA FLUOR 647

Synonyms

H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX

Long name

Histone H2A.X (Ser140) Antibody, ALEXA FLUOR 647 Conjugated

Specificity

This is a highly specific antibody against Histone H2A.X Ser140.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-Histone H2A.X Ser140 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.