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Providers / Bioss Primary Conjugated Antibodies. ALEXA FLUOR / Crk II (Tyr221) Antibody, ALEXA FLUOR 488

Description:

Size: 100ul

Catalog no.: bs-3121R-A488

Price: 350 EUR

Get for 350 EUR on Gentaur.com

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Product details

Gene ID Number

1398

Modification Site

Tyr221

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Conjugation

Alexa Fluor

Excitation emission

499nm/519nm

Concentration

1ug per 1ul

Modification

Phosphorylation

Crossreactivity

Human, Mouse, Rat

Conjugated with

ALEXA FLUOR® 488

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Target Antigen

Crk isoform II Tyr221

Purification

Purified by Protein A.

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Crk isoform II Tyr221 PAb ALEXA FLUOR 488

Synonyms

Adapter molecule crk; Proto-oncogene c-Crk; p38; CRK

Long name

Crk II (Tyr221) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Specificity

This is a highly specific antibody against Crk isoform II Tyr221.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP(p-Y)AQ]

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Background of the antigen

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Crk isoform II Tyr221 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.