Product details

Modification site

None

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Target Protein/Peptide

PAPOL A+B+G

Excitation emission

590nm/617nm

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Gene ID

10914, 56903, 64895

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Swiss Prot

P51003, Q9NRJ5, Q9BWT3

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Other name

Anti-PAPOL A+B+G Polyclonal

Subcellular locations

Cytoplasm, Nucleus, Lumenal

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

PAPOL A+B+G Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with PAPOL A+B+G

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

PAPOL A/B/G; PAP alpha/beta/gamma; PapIII; MGC5378; PAP alpha; PAP beta; PAP gamma; PAP; PAP-alpha; PAP-beta; PAP-gamma; PAPOLA; PAPOLB; PAPOLG; PAPOA_HUMAN; PAPOB_HUMAN; PAPOG_HUMAN; Plap; polyA polymerase alpha; Polynucleotide adenylyltransferase alpha; polyA polymerase beta testis specic; polyA polymerase beta; polynucleotide adenylyltransferase beta; TPAP; Testis-specic polyA polymerase.

Background information

Polyadenylation of the 3-prime ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site that leads to synthesis of the poly(A) tail at the 3-prime end of the upstream cleavage product. The poly(A) polymerase (PAP) is required The adenosine addition reaction depends on poly(A) polymerase (PAP) activity. The testis express PAP-beta (TPAP) in the cytoplasm of spermatogenic cells. The adenosine addition function of PAP-beta plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice display impaired expression of haploid-specific genes that are necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3.

Anti-PAPOL A+B+G (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-9435R-A594

Price: 489 EUR