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Providers / Bioss Polyclonal Antibodies / Anti-Histone H2A.X (Ubiquityl Lys119), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-8571R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Modification site

None

Gene ID

3014

Swiss Prot

P16104

Subcellular locations

Nucleus

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Target Protein/Peptide

Histone H2A.X Ubiquityl Lys119

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-Histone H2A.X (Ubiquityl Lys119)

Also known as

Histone H2A.X (Ubiquityl Lys119) Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Rat (Rattus norvegicus)

Modification

No modification has been applied to this antibody

Synonyms

H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX

Antigen Source

KLH conjugated synthetic peptide derived from human Ubiquityl Histone H2A.X (Lys119)

Specificity

This modification site is homologous to that of Lys119 in Rat. Histone H2A.X (Ubiquityl Lys119)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.