KLH conjugated synthetic peptide derived from human ATP5E
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
ATP 5E; ATP synthase epsilon chain mitochondrial; ATP synthase H+ transporting mitochondrial F1 complex epsilon subunit; ATP synthase subunit epsilon; ATP5E; ATP5E_HUMAN; ATPase subunit epsilon; ATPE; F0F1 ATPase; H+ transporting two sector ATPase; MGC104243; mitochondrial; Mitochondrial ATP synthase epsilon chain; Mitochondrial ATPase.
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one e chain. F0 consists of three subunits: a, b and c. The e chain of F1 contains 50 amino acids and is the smallest of the five ATPase F1 chains. Mitochondrial ATPase e chain (ATP5E) localizes to the mitochondria and catalyzes ATP synthesis.