Product details

Gene ID

999

Modification site

None

Swiss Prot

P12830

Target Protein/Peptide

E cadherin

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Subcellular locations

Extracellular

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Applications

FCM, IF(IHC-P), IF(ICC)

Group

Polyclonals and antibodies

Other name

Anti-E cadherin Polyclonal

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Also known as

E cadherin Polyclonal Antibody

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with E cadherin

Modification

No modification has been applied to this antibody

Applications with corresponding dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Antigen Source

KLH conjugated synthetic peptide derived from human E-cadherin

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Synonyms

UVO; CDHE; ECAD; LCAM; Arc-1; CD324; Cadherin-1; CAM 12/8; Epithelial cadherin; E-cadherin; Uvomorulin; CDH1

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Anti-E cadherin (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-10009R-A594

Price: 489 EUR