Product details

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

N/A

Modification site

None

Cross reactive species

Target Protein/Peptide

His tagCT

Applications

IF(IHC-P)

Cross Reactive Species details

HHHHHH tag

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Other name

Anti-His tag(CT) Polyclonal

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

His tag(CT) Polyclonal Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594,histidine

Properties

For facs or microscopy Alexa 1 conjugate.

Antigen Source

synthetic peptide: HHHHHH conjugated to KLH

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with His tag(CT)

Synonyms

HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Anti-His tag(CT) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-0899R-A594

Price: 489 EUR