Product details

Swiss Prot

N/A

Gene ID

N/A

Subcellular locations

N/A

Target Protein/Peptide

EGFP

Modification site

None

Cross reactive species

Others

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Cross Reactive Species details

EGFP protein

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Other name

Anti-EGFP Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

EGFP Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Synonyms

Enhanced Green Fluorescent Protein.

Properties

For facs or microscopy Alexa 1 conjugate.

Modification

No modification has been applied to this antibody

Antigen Source

Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria

Specificity

This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria.EGFP

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Conjugate

The Green Fluorescent Protein (eGFP) of Anti-EGFP can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.

Anti-EGFP (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-2194R-A594

Price: 489 EUR