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Providers / Bioss Polyclonal Antibodies / Anti-Acetyl-Histone H4(K12), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3746R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Modification site

K12

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

121504

Applications

IF(IHC-P)

Modification

Acetylation

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Target Protein/Peptide

Acetyl-Histone H4K12

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Type

Conjugated Primary Antibody

Other name

Anti-Acetyl-Histone H4(K12)

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Acetyl-Histone H4(K12) Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with Acetyl-Histone H4(K12)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic acetylpeptide derived from human Histone H4 around acetylation site of Lys12

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

Histone H4 Acetyl K12; Histone H4 Acetyl Lys12; Acetyl-Histone H4 Lys12; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Description

N terminal acetylation or CH3CO as epigenetic regulation of Anti-Acetyl-Histone H4(K12) by NATs.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].