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Providers / Bioss Polyclonal Antibodies / Anti-EEF2 (Thr56 + Thr58) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-13057R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Swiss Prot

N/A

Subcellular locations

N/A

Gene ID

1938

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Modification site

Thr56 + Thr58

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Target Protein/Peptide

EEF2 Thr56 + Thr58

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-EEF2 (Thr56 + Thr58) Polyclonal

Also known as

EEF2 (Thr56 + Thr58) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with EEF2 (Thr56 + Thr58)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human EEF2 around the phosphorylation site of Thr56+Thr58

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

EEF2 phospho T56 + T58; p-EEF2 phospho T56 + T58; EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Background information

EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.