Product details

Subcellular locations

N/A

Gene ID

N/A

Swiss Prot

N/A

Modification site

None

Target Protein/Peptide

PARD6G

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Other name

Anti-PARD6G Polyclonal

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Also known as

PARD6G Polyclonal Antibody

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with PARD6G

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human PARD6G

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

PAR 6 gamma protein; par 6 partitioning defective 6 homolog gamma; PAR 6G; PAR6D; PAR6gamma; Partitioning defective 6 homolog gamma.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

PARD6G (partitioning defective 6 homolog gamma) is a 376 amino acid adaptor protein that is involved in cell polarization and asymmetrical cell division processes. PARD6G contains one OPR domain, one PDZ (DHR) domain and one pseudo-CRIB domain. The PDZ and pseudo-CRIB domains are required for interaction with Rho small GTPases. Through its complex formation with PARD3G, PARD6G participates in the linking of GTP-bound Rho small GTPases to atypical protein kinase C (PKC) proteins. This assembly is involved in formation of normal tight junctions at epithelial cell-cell contacts. When atypical PKC and PARD6G are expressed with a constitutively active RAC, the proteins colocalize to the membrane ruffles, which are structures that occur at the leading edge of polarized cells during movement. Though widely expressed, PARD6G is found at highest levels in adult and fetal kidney.

Anti-PARD6G (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-13696R-A594

Price: 489 EUR