Product details

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

8706

Modification site

None

Target Protein/Peptide

B3GALNT1

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-B3GALNT1 Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Also known as

B3GALNT1 Polyclonal Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with B3GALNT1

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human B3GALNT1

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. Multiple transcript variants that are alternatively spliced in the 5' UTR have been described; they all encode the same protein.

Synonyms

3-galactosyltransferase 3; 3-GalNAc-T1; 3-GalTase 3; 3-N-acetylgalactosaminyltransferase 1; 3-N-acetylgalactosaminyltransferase; b3Gal T3; b3Gal-T3; B3galnt1; B3GALT3; B3GL1_HUMAN; Beta 1,3 galactosyltransferase 3; Beta 1,3 GalTase 3; beta 1,3 N acetylgalactosaminyltransferase 1 globoside blood group; Beta 3 GalNAc T1; Beta 3 Gx T3; Beta-1; Beta-3-Gx-T3; beta3Gal T3; Beta3Gal-T3; Beta3GalT3; brainiac1; Galactosylgalactosylglucosylceramide beta D acetyl galactosaminyltransferase; Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase; galT3; Gb4Cer; GLCT3; GLOB; Globoside synthase; globotriaosylceramide 3 beta N acetylgalactosaminyltransferase; P antibody P antigen synthase; P blood group globoside; P1 antibody UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase 1; UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase, polypeptide 3 Globoside blood group; UDP GalNAc:beta 1,3 N acetylgalactosaminyltransferase 1; UDP GalNAc:betaGlcNAc beta 1,3 galactosaminyltransferase, polypeptide 1 Globoside blood group; UDP N acetylgalactosamine:globotriaosylceramide beta 1,3 N acetylgalactosaminyltransferase; UDP-GalNAc:beta-1; UDP-N-acetylgalactosamine:globotriaosylceramide beta-1

Anti-B3GALNT1 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-10438R-A594

Price: 489 EUR