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Providers / Bioss Polyclonal Antibodies / Anti-ASAP1 (Tyr782) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-5176R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Swiss Prot

N/A

Subcellular locations

N/A

Gene ID

13196

Modification site

Tyr782

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

ASAP1 Tyr782

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-ASAP1 (Tyr782) Polyclonal

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

ASAP1 (Tyr782) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with ASAP1 (Tyr782)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from mouse DDEF1 around the phosphorylation site of Tyr782

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Synonyms

ASAP1 Tyr782; ASAP1 Y782; p-ASAP1 Tyr782; p-ASAP1 Y782; ASAP1 / DDEF1; AMAP 1; AMAP1; AMAP-1; ARF GTPase activating protein 1; ASAP 1; Centaurin beta 4; CentB4; DDEF 1; DDEF1 protein; PAG 2; PAG2; PAG-2; PAP; PIP2 dependent ARF1 GAP; ZG 14P; ZG14P; Development and dferentiation enhancing factor 1; 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein; ADP ribosylation factor directed GTPase activating protein 1ï¼›ASAP1_HUMAN.