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Providers / Bioss Polyclonal Antibodies / Anti-RBM15/SPEN (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-12402R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Subcellular locations

N/A

Gene ID

N/A

Swiss Prot

N/A

Modification site

None

Applications

IF(IHC-P)

Target Protein/Peptide

RBM15/SPEN

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-RBM15/SPEN Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Also known as

RBM15/SPEN Polyclonal Antibody

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with RBM15/SPEN

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human RBM15

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Synonyms

One twenty two protein; OTT; OTT1; SPEN; RNA binding mot protein 15; RBM15_HUMAN.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

RBM15 is a 977 amino acid protein that localizes to the nucleus and contains one SPOC domain and three RRM domains. Expressed as multiple alternatively spliced isoforms, RBM15 interacts with Epstein-Barr (EBV) viral proteins and is thought to be involved in the regulation of Hox genes, possibly via interactions with RNA and spliceosome components. RBM15 is subject to post-translational phosphorylation, probably by ATM or ATR. Chromosomal aberrations involving the RBM15 gene, which localizes to human chromosome 1, may be associated with the development of acute megakaryoblastic leukemia.