Product details

Swiss Prot

N/A

Modification site

None

Gene ID

3135

Target Protein/Peptide

HLA G

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Subcellular locations

Extracellular

Applications

FCM, IF(IHC-P)

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Cross reactive species

Human (Homo sapiens)

Clone

Polyclonal Antibodies

Other name

Anti-HLA G Polyclonal

Purification method

Purified by Protein A.

Also known as

HLA G Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Applications with corresponding dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with HLA G

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human of human HLA-G

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Synonyms

B2 microglobulin; DADB-15K14.8; HLA 6.0; HLA 60; HLA class I histocompatibility antigen alpha chain G; HLA class I histocompatibility antigen alpha chain G precursor; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G; HLA G antigen; HLA G histocompatibility antigen class I G; HLA G protein; HLA G2.2; HLA G3; HLA-G; HLA-G histocompatibility antigen, class I; HLA60; HLAG; HLAG_HUMAN; Human Leukocyte Antigen G Histocompatability Antigen Class 1; Lymphocyte antigen; Major histocompatibility complex class I G; MHC class I antigen; MHC class I antigen G; MHC class Ib antigen; MHC G; Murine homolog of Qa; T Cell A Locus; T-cell A locus; TCA.

Anti-HLA G (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-0752R-A594

Price: 489 EUR