Description:

    Size: 100 microliters

    Catalog no.: GENTObs-1607R-A594

    Price: 489 EUR

    Product details

    Gene ID

    1457

    Modification site

    None

    Swiss Prot

    P68400

    Applications

    IF(IHC-P)

    Target Protein/Peptide

    CK II alpha

    Excitation emission

    590nm/617nm

    Concentration

    1ug per 1ul

    Conjugated

    Alexa conjugate 1

    Conjugated with

    ALEXA FLUOR® 594

    Subcellular locations

    Cytoplasm, Nucleus

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clonality

    Polyclonal Antibody

    Clone

    Polyclonal Antibodies

    Purification method

    Purified by Protein A.

    Group

    Polyclonals and antibodies

    Type

    Conjugated Primary Antibody

    Other name

    Anti-CK II alpha Polyclonal

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    CK II alpha Polyclonal Antibody

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Modification

    No modification has been applied to this antibody

    Specificity

    This antibody reacts specifically with CK II alpha

    Antigen Source

    KLH conjugated synthetic peptide derived from human CK II alpha

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

    Synonyms

    CKII; CK2A1; CSNK2A3; Casein kinase II subunit alpha; CK II alpha; CSNK2A1

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Description

    The Anti-CK II alpha is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

    Background information

    Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation.