Product details

Target Protein/Peptide

CD8

Modification site

None

Gene ID

12525

Swiss Prot

P01731

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Subcellular locations

Extracellular

Applications

FCM, IF(IHC-P)

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Other name

Anti-CD8 Polyclonal

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

CD8 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Applications with corresponding dilutions

FCM(1:20-100), IF(IHC-P)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with CD8

Cross reactive species

Mouse (Mus musculus), Rat (Rattus norvegicus)

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from mouse CD8

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Lyt2

Background information

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Anti-CD8 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-0648R-A594

Price: 489 EUR