Product details

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

4172

Target Protein/Peptide

MCM3

Modification site

None

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Other name

Anti-MCM3 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

MCM3 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with MCM3

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human MCM3

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

minichromosome maintenance deficient 3; Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; MCM3 minichromosome maintenance deficient 3; MGC1157; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1.h; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB.

Background information

MCM3 is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Anti-MCM3 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-1982R-A594

Price: 489 EUR