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    Providers / Bioss Polyclonal Antibodies / Anti-Collagen III (Polyclonal), ALEXA Fluor 594

    Description:

    Size: 100 microliters

    Catalog no.: GENTObs-0948R-A594

    Price: 489 EUR

    Get for 489 EUR on Gentaur.com

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    Product details

    Gene ID

    1281

    Modification site

    None

    Swiss Prot

    P02461

    Subcellular locations

    Secreted

    Applications

    IF(IHC-P)

    Excitation emission

    590nm/617nm

    Concentration

    1ug per 1ul

    Target Protein/Peptide

    Collagen III

    Conjugated with

    ALEXA FLUOR® 594

    Conjugated

    Alexa conjugate 1

    Clonality

    Polyclonal Antibody

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clone

    Polyclonal Antibodies

    Purification method

    Purified by Protein A.

    Group

    Polyclonals and antibodies

    Type

    Conjugated Primary Antibody

    Other name

    Anti-Collagen III Polyclonal

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    Collagen III Polyclonal Antibody

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Synonyms

    EDS4A; Collagen alpha-1(III) chain; COL3A1

    Modification

    No modification has been applied to this antibody

    Specificity

    This antibody reacts specifically with Collagen III

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

    Antigen Source

    KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain

    Gene

    Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    Description

    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Background information

    Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.