Product details

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

6928

Modification site

None

Target Protein/Peptide

HNF1 beta

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-HNF1 beta Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Also known as

HNF1 beta Polyclonal Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with HNF1 beta

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human HNF1 beta

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

FJHN; Hepatocyte nuclear factor 1 beta; Hepatocyte nuclear factor 1-beta; HNF 1B; HNF 2; HNF-1-beta; HNF-1B; HNF1B; HNF1 beta; HNF1 homeobox B; HNF1B; HNF1B_HUMAN; HNF1beta; HNF2; Homeoprotein LF B3; Homeoprotein LFB3; HPC11; LF B3; LFB3; MODY 5; MODY5; TCF 2; TCF 2 protein; TCF-2; TCF2; TCF2 protein; Transcription factor 2; Transcription factor 2 hepatic; Variant hepatic nuclear factor 1; Variant hepatic nuclear factor; VHNF 1; vHNF1.

Background information

HNF-1 (a and b), HNF-3 (a, b and g), HNF-4 (a and g) and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3a, HNF-3b and HNF-3g regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3a and HNF-3b have also been shown to be involved in gastrulation events such as body axis formation. HNF-4a and HNF-4g have been shown to be important for early embryo development. HNF-4a is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4g is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3b, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and a-1 antitrypsin.

Anti-HNF1 beta (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-8617R-A594

Price: 489 EUR