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Providers / Bioss Polyclonal Antibodies / Anti-Apolipoprotein O (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-12502R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Swiss Prot

N/A

Subcellular locations

N/A

Modification site

None

Gene ID

79135

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

Apolipoprotein O

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-Apolipoprotein O Polyclonal

Also known as

Apolipoprotein O Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with Apolipoprotein O

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic peptide derived from human ApoO/Apolipoprotein O

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

ApoO; 0610008C08Rik; 1110019O03Rik; Apolipoprotein O; APOO; APOO_HUMAN; FAM121B; Family with sequence similarity 121B; MGC130105; MGC130106; MGC4825; My025; MYO25; OTTHUMP00000023073; Protein FAM121B; RP23-272D10.2.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Apolipoproteins are a family of fatty-acid binding proteins that transport fat through the bloodstream in the form of lipoproteins. ApoO (Apolipoprotein O), also known as FAM121B or My025, is a 198 amino acid single-pass membrane protein that belongs to the apolipoprotein family. Expressed ubiquitously with particularly high expression in diabetic heart tissue, apoO functions to promote the transport of cholesterol from macrophage cells and may be involved in regulatory mechanisms that protect lipid accumulation within the heart. ApoO is present in high density lipoproteins (HDLs) and low density lipoproteins (LDLs) and is secreted by an MTP (microsomal triglyceride transfer protein)-dependent mechanism. Two isoforms of apoO exist due to alternative splicing events.