Product details

Gene ID

13666

Swiss Prot

Q9Z2B5

Modification site

Thr980

Subcellular locations

Cytoplasm

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

PERK Thr980

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications

IF(IHC-P), IF(ICC)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Other name

Anti-PERK(Thr980) Polyclonal

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

PERK(Thr980) Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with PERK(Thr980)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr981

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

Pek; Perk; AI427929; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; Eif2ak3

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Anti-PERK(Thr980) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3330R-A594

Price: 516 EUR