Product details

Subcellular locations

N/A

Gene ID

13449

Swiss Prot

O70469

Modification site

Tyr351

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Target Protein/Peptide

DOK2 Tyr351

Excitation emission

590nm/617nm

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-DOK2 (Tyr351) Polyclonal

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

DOK2 (Tyr351) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from mouse p56Dok2 around the phosphorylation site of (Tyr351)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

DokR; Frip; Docking protein 2; Dok-related protein; Dok-R; Downstream of tyrosine kinase 2; IL-four receptor-interacting protein; p56(dok-2); Dok2

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Specificity

This phosphorylation site is found at Tyr345 in Human. Due to the highly conserved nature of this sequence with DOK1, this antibody may react with DOK1 when it is phosphorylated at Tyr362 in Human and Tyr361 in Mouse and Rat.DOK2 (Tyr351)

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Background information

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Anti-DOK2 (Tyr351) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3101R-A594

Price: 516 EUR