Product details

Gene ID

12125

Modification site

Ser55

Swiss Prot

O54918

Applications

IF(IHC-P)

Target Protein/Peptide

Bim Ser55

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Subcellular locations

Cytoplasm, Membrane bound

Other name

Anti-Bim(Ser55) Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Bim(Ser55) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Specificity

This phosphorylation site is homologous to Ser59 in Human and Ser55 in Rat.Bim(Ser55)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from mouse Bim around the phosphorylation site of Ser55

Synonyms

Bim; Bod; bcl2-L-11; 1500006F24Rik; Bcl-2-like protein 11; Bcl2-interacting mediator of cell death; Bcl2l11

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Background information

Induces apoptosis and anoikis. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Anti-Bim(Ser55) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3058R-A594

Price: 516 EUR