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Providers / Bioss Polyclonal Antibodies / Anti-KALRN/Duo (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-11861R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Subcellular locations

N/A

Gene ID

N/A

Swiss Prot

N/A

Modification site

None

Target Protein/Peptide

KALRN/Duo

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-KALRN/Duo Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Also known as

KALRN/Duo Polyclonal Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with KALRN/Duo

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human KALRN/Duo

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Synonyms

Duo; FLJ16443; HAPIP; Huntingtin associated protein interacting protein duo ; Huntingtin-associated protein-interacting protein; Kalirin isoform 2; Kalirin; KALRN ; KALRN_HUMAN ; Protein Duo; RhoGEF kinase; Serine/threonine kinase with Dbl and pleckstrin homology domains ; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain; TRAD.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

HAP1 (huntingtin-associated protein 1) binds to huntingtin (1). Huntingtin is a protein that contains a polyglutamine region and when the number of glutamine repeats exceeds 35, the gene encodes a version of huntingtin that leads to Huntington’s disease (HD) (2,3). The ability of HAP1 to bind to huntingtin is enhanced by an expanded polyglutamine repeat region (1). HAP1 shows neuronal localization and moves with huntingtin in nerve fibers (4,5). HAP1 is primarily expressed in brain tissue, with greater expression in the olfactory bulb and brain stem (1). Mouse HAP1 is localized to membrane-bound organelles including large endosomes, tubulovesicular structures and budding vesicles in neurons (6). Duo, also designated huntingtin-associated protein interacting protein or HAPIP, binds Huntingtin-associated protein 1 (HAP1) and may have a role in vesicle trafficking and cytoskeletal function.