Product details

Gene ID

24224

Modification site

Ser70

Swiss Prot

P49950

Subcellular locations

Cytoplasm

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

Bcl-2 Ser70

Modification

Phosphorylation

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications

IF(IHC-P), IF(ICC)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-Bcl-2 (Ser70) Polyclonal

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Bcl-2 (Ser70) Polyclonal Antibody

Synonyms

Bcl-2; Apoptosis regulator Bcl-2; Bcl2

Applications with corresponding dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Mouse (Mus musculus), Rat (Rattus norvegicus)

Specificity

This antibody reacts specifically with Bcl-2 (Ser70)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from rat Bcl-2 around the phosphorylation site of Ser70

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Anti-Bcl-2 (Ser70) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3052R-A594

Price: 516 EUR