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Providers / Bioss Polyclonal Antibodies / Anti-4 Hydroxynonenal (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-6313R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

N/A

Modification site

None

Cross reactive species

Others

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Target Protein/Peptide

4 Hydroxynonenal

Cross Reactive Species details

4-Hydroxynonenal

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-4 Hydroxynonenal Polyclonal

Antigen Source

4 Hydroxynonenal conjugated to BSA

Also known as

4 Hydroxynonenal Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Modification

No modification has been applied to this antibody

Specificity

This antibody reacts specifically with 4 Hydroxynonenal

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Synonyms

4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; 4 HNE; 4HNE; 4-HNE; E-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion.