Product details

Subcellular locations

N/A

Swiss Prot

N/A

Target Protein/Peptide

ARL4

Modification site

None

Gene ID

10124

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Other name

Anti-ARL4 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

ARL4 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with ARL4

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human ARL4

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Synonyms

ADP ribosylation factor like 4A; ADP-ribosylation factor-like protein 4A; ARL 4; ARL4A; ARL4A_HUMAN.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

ADP-ribosylation factors (ARFs) are highly conserved guanine nucleotide-binding proteins that enhance the ADP-ribosyltransferase activity of cholera toxin. ARFæŠ¯ are important in eukaryotic vesicular trafficking pathways and activating phospholipase D. ARL4 (ADP-ribosylation factor-like protein 4A) is a member of the ARF-like protein (ARL) subfamily of small GTPases. It contains a C terminal nuclear localization signal (NLS) region that interacts with Importin-? ARL4 localizes to the nucleus and is found in a variety of tissues, but is predominantly expressed in spermatogonia and Sertoli cells. It is most closely related to ARL6 and ARL7. Unlike ARFs, ARL4 does not activate the cholera toxin ADP-ribosyltranferase. ARL4 may play a role in neurogenesis during embryonic development and somitogenesis in the early stages of adult spermatogenesis.

Anti-ARL4 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-12523R-A594

Price: 489 EUR