Description:

Size: 100 microliters

Catalog no.: GENTObs-11424R-A594

Price: 489 EUR

Product details

Swiss Prot

N/A

Gene ID

N/A

Subcellular locations

N/A

Modification site

None

Applications

IF(IHC-P)

Target Protein/Peptide

Sauvagine

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Cross reactive species

Other Species

Cross Reactive Species details

Xenopus laevis

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-Sauvagine Polyclonal

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Also known as

Sauvagine Polyclonal Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Synonyms

Sauvagin; Sauvagine; SVG; SAUV_PHYSA.

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with Sauvagine

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from frog Sauvagine (C-QGPPISIDLSLELLRKMIEIEKQEKEKQQAANNRLLLDTI-NH2)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Sauvagine is a 40-residue peptide isolated and characterized from frog skin. It shares significant structural homology with and elicits similar biological responses as corticotropin-releasing factor (CRF). It has high affinity for both the CRF1 and CRF2 receptors (and low affinity for the CRF binding protein), suggesting its potential as a pharmacological tool for the study of these receptor sub-types. Due to the binding to CRF2 receptors and their role in the release of ACTH and beta-endorphin during stress, possible areas of research with this ligand include anxiety, depression and obesity.