0208080893 [email protected]
Providers / Bioss Polyclonal Antibodies / Anti-EYA1 (Polyclonal), ALEXA Fluor 647

Description:

Size: 100 microliters

Catalog no.: GENTObs-20158R-A647

Price: 489 EUR

Get for 489 EUR on Gentaur.com

/ningbo-assets-bio/graphics/prods/bigs/bigs3.jpg

Product details

Target Protein/Peptide

EYA1

Modification site

None

Gene ID

2138

Swiss Prot

Q99502

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Excitation emission

650nm/665nm

Conjugated with

ALEXA FLUOR® 647

Subcellular locations

Cytoplasm, Nucleus

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR 647

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-EYA1 Polyclonal ALEXA FLUOR® 647

Also known as

EYA1 Polyclonal Antibody ALEXA FLUOR® 647

Cross reactive species

Human (Homo sapiens), Rat (Rattus norvegicus)

Synonyms

BOP; BOR; BOS1; OFC1; Eyes absent homolog 1; EYA1

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human EYA1

Specificity

This antibody reacts specifically with EYA1 ALEXA FLUOR® 647

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Polyclonal Antibodies made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-EYA1 ALEXA FLUOR® 647 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.

Background information

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2.