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Providers / Bioss Polyclonal Antibodies / Anti-Histone H3 (acetyl K9), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3776R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Gene ID

3020

Swiss Prot

P84243

Subcellular locations

Nucleus

Modification site

acetyl K9

Applications

IF(IHC-P)

Modification

Acetylation

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Target Protein/Peptide

Histone H3 acetyl K9

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Type

Conjugated Primary Antibody

Other name

Anti-Histone H3 (acetyl K9)

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Histone H3 (acetyl K9) Antibody

Synonyms

H3F3; H3.3A; Histone H3.3; H3F3A; PP781

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Specificity

This acetylation site is homologous across the species listed. Histone H3 (acetyl K9)

Antigen Source

KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Description

N terminal acetylation or CH3CO as epigenetic regulation of Anti-Histone H3 (acetyl K9) by NATs.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.