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Providers / Bioss Polyclonal Antibodies / Anti-Cyclin B1 (Ser147) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3122R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Gene ID

891

Swiss Prot

N/A

Subcellular locations

N/A

Modification site

Ser147

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Modification

Phosphorylation

Target Protein/Peptide

Cyclin B1 Ser147

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-Cyclin B1 (Ser147) Polyclonal

Also known as

Cyclin B1 (Ser147) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with Cyclin B1 (Ser147)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Synonyms

Cyclin B1 phospho S147; Cyclin B1 Ser147; p-Cyclin B1 S147; p-Cyclin B1 Ser147; p-Cyclin B1 phospho S147; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].