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Providers / Bioss Polyclonal Antibodies / Anti-Ki-67 (Proliferation Marker), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-2130R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Gene ID

4288

Modification site

None

Swiss Prot

P46013

Subcellular locations

Nucleus

Concentration

1ug per 1ul

Excitation emission

590nm/617nm

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications

IF(IHC-P), IF(ICC)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Target Protein/Peptide

Ki-67 proliferation Marker

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Other name

Anti-Ki-67 (Proliferation Marker)

Also known as

Ki-67 (Proliferation Marker) Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus)

Synonyms

KIA; MIB-; MIB-1; PPP1R15; Antigen KI-67; MKI67

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human Ki67

Specificity

This antibody reacts specifically with Ki-67 (Proliferation Marker)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.