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Providers / Bioss Polyclonal Antibodies / Anti-SSEA3 (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-3575R-A594

Price: 489 EUR

Get for 489 EUR on Gentaur.com

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Product details

Gene ID

N/A

Swiss Prot

N/A

Modification site

None

Target Protein/Peptide

SSEA3

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Subcellular locations

Cell membrane

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications

IF(IHC-P), IF(ICC)

Clonality

Polyclonal Antibody

Clone

Polyclonal Antibodies

Other name

Anti-SSEA3 Polyclonal

Purification method

Purified by Protein A.

Also known as

SSEA3 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Antigen Source

4-8 cell stage mouse embryos

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Applications with corresponding dilutions

IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with SSEA3

Cross reactive species

Human (Homo sapiens), Rat (Rattus norvegicus)

Modification

No modification has been applied to this antibody

Synonyms

SSEA-3; Stage specic embryonic antigen 3; Stage specic embryonic antigen 3.

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Background information

SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.