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Providers / Bioss Polyclonal Antibodies / Anti-EZH2 (Thr487) (Polyclonal), ALEXA Fluor 594

Description:

Size: 100 microliters

Catalog no.: GENTObs-6529R-A594

Price: 516 EUR

Get for 516 EUR on Gentaur.com

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Product details

Swiss Prot

N/A

Subcellular locations

N/A

Gene ID

2146

Modification site

Thr487

Applications

IF(IHC-P)

Concentration

1ug per 1ul

Target Protein/Peptide

EZH2 Thr487

Excitation emission

590nm/617nm

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 594

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Other name

Anti-EZH2 (Thr487) Polyclonal

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

EZH2 (Thr487) Polyclonal Antibody

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with EZH2 (Thr487)

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human KMT6 around the phosphorylation site of Thr487 [VD(p-T)P]

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

KMT6/EZH2phospho T487; KMT6/EZH2phospho Thr487; p-KMT6/EZH2phospho T487; p-KMT6/EZH2Thr487; p-KMT6/EZH2T487; Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH1; EZH2; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2 Drosophila; Enhancer of zeste, Drosophila, homolog 2; KMT 6; KMT6; KMT6A; WVS2.

Background information

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011].