Description:

    Size: 100 microliters

    Catalog no.: GENTObs-7833R-A594

    Price: 516 EUR

    Product details

    Swiss Prot

    N/A

    Subcellular locations

    N/A

    Gene ID

    8881

    Modification site

    Ser560

    Applications

    IF(IHC-P)

    Concentration

    1ug per 1ul

    Excitation emission

    590nm/617nm

    Target Protein/Peptide

    CDC16 Ser560

    Modification

    Phosphorylation

    Conjugated with

    ALEXA FLUOR® 594

    Conjugated

    Alexa conjugate 1

    Clonality

    Polyclonal Antibody

    Applications with corresponding dilutions

    IF(IHC-P)(1:50-200)

    Clone

    Polyclonal Antibodies

    Purification method

    Purified by Protein A.

    Group

    Polyclonals and antibodies

    Type

    Conjugated Primary Antibody

    Other name

    Anti-CDC16(Ser560) Polyclonal

    Conjugation

    Alexa Fluor,ALEXA FLUOR® 594

    Host organism

    Rabbit (Oryctolagus cuniculus)

    Also known as

    CDC16(Ser560) Polyclonal Antibody

    Properties

    For facs or microscopy Alexa 1 conjugate.

    Specificity

    This antibody reacts specifically with CDC16(Ser560)

    Cross reactive species

    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

    Antigen Source

    KLH conjugated synthetic phosphopeptide derived from human APC6 around the phosphorylation site of Ser560

    Storage

    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

    Description

    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

    About

    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

    Cross Reactive Species details

    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

    Advisory

    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

    Synonyms

    Apc6 phospho S560; p-Apc6 phospho S560; ANAPC6; Anaphase promoting complex subunit 6; Anaphase-promoting complex subunit 6; Apc 6; APC6; CDC 16; Apc6 phospho S560: p-Apc6S560; CDC16 cell division cycle 16 S. cerevisiae homolog; CDC16; CDC16 homolog; CDC16 protein; CDC16_HUMAN; CDC16Hs; Cell division cycle 16 homolog; Cell division cycle protein 16 homolog; CUT9; Cyclosome subunit 6.

    Background information

    Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.